Analysis of gallic acid and ellagic acid in leaves of Elaeagnus angustifolia L. From different habitats and times in Xinjiang by HPLC with cluster analysis

Abstract

Objective: To determine the levels of gallic acid and ellagic acid by using high-performance liquid chromatography (HPLC) with R software hierarchical cluster analysisin Elaeagnus angustifolia L. gathered from different locations in Xinjiang. Methods: A chromatographic column Diamonsil C18 with a size of 4.6 3 250 mm and 5 mm was used with methanol as A and 0.1% phosphoric acid aqueous solution as B as the mobile phase. The flow rate was 1 mL/min for the gradient elution and the injection volume was 5 mL. HPLC was performed with a detection wavelength of 260 nm and chromatographic column of 35 8C. In addition, R software hierarchical clustering method was used for studying the levels of gallic acid and ellagic acid in E. angustifolia L. from 10 areas. Results: Gallic acid and ellagic acid showed a good linear relationship between 7.375 and 236 mg/mL with a correlation coefficient of 0.9999, and between 3.625 and 116 mg/mL with a correlation coefficient of 0.9999 respectively. The average recovery values were 103.98 and 101.57%, and the Relative Standard Deviation (RSD) values were 1.92 and 1.47%. Conclusion: Differences in the levels of gallic acid and ellagic acid in E. angustifolia L. leaves from different areas in Xinjiang showed that both were the highest in Kuitun.