Complete sequence of broad-host-range plasmid pRIO-5 harboring the extended-spectrum-β-lactamase gene blaBES-1

Abstract

Broad-host-range plasmid pRIO-5, harboring the extended-spectrum β-lactamase blaBES-1 gene in Serratia marcescens, was fully sequenced. Analysis of the 12,957-bp sequence of this IncP6-type plasmid revealed that the blaBES-1 gene was associated with two copies of the insertion sequence IS26. The promoter responsible for the blaBES-1 expression was hybrid, made of a -35 box located inside the inverted repeat of IS26 and a -10 box inside a remnant of an insertion sequence. Copyright © 2012, American Society for Microbiology. All Rights Reserved.