Hemato-Biochemical Alterations in Water Buffaloes Clinically Infected with Bovine Theileriosis before and after tTreatment by Buparvaquone

Abstract

Most of research about theileriosis have been done about its effect in cows and derelict it in water buffaloes. So this investigation is aimed to study the hematological and serum constituents alterations in buffaloes affected with bovine theileriosis in Egypt. A total of thirty six buffaloes about 3-5 years old were examined in this study. Twenty six were naturally infected by Theileria. Another ten buffaloes of the same age and apparent healthy were kept as control. The infected buffaloes were treated by buparvaquone (50 mg/20 kg B.W). Blood were taken from the healthy, infected non treated buffaloes and then post one and two weeks from buparvaquone administration. The affected buffaloes revealed high fever, corneal opacity, lacrimation, enlargement of the prescapular lymph node. Blood smears confirm presence of piroplasms of T. annulata in red blood corpuscles. Hematological analysis showed a significant reduction in erythrocytes count (RBCs), packed cell volume (PCV) and hemoglobin concentration (Hb) in non treated buffaloes infected by Theileria. Normocytic normochromic anemia was reported rapidly with the first appearance of signs. Latterly, macrocytic hypochromic anemia. Total leucocyte count significantly increased soon after infection and persists until the first week post buparvaquone treatment. Theileria infected non treated buffaloes revealed an increase in the serum activities of aminotransferase (AST and ALT). Also serum levels of total bilirubin, unconjugated bilirubin (UB), conjugated bilirubin (CB), creatinine and blood urea nitrogen level were increased. In the opposite, side, the total serum proteins and albumin levels significantly decreased. Significant increase in malonyldialdehyde (MDA) activity was recorded with a significant decease in the catalase activity (CAT) in T. annulata clinically infected buffaloes. These alterations were improved by treatment of the diseased buffaloes by buparvaquone two weeks post administration.