Petrifilm™ Rapid S. aureus count plate method for rapid enumeration of Staphylococcus aureus in selected foods: Collaborative study

Abstract

A rehydratable dry-film plating method for Staphylococcus aureus in foods, the 3M™ Petrifilm™ Rapid S. aureus Count Plate method, was compared with AOAC® Official Method(Service mark) 975.55 (Staphylococcus aureus in Foods). Nine foods - instant nonfat dried milk, dry seasoned vegetable coating, frozen hash browns, frozen cooked chicken patty, frozen ground raw pork, shredded cheddar cheese, fresh green beans, pasta filled with beef and cheese, and egg custard - were analyzed for S. aureus by 13 collaborating laboratories. For each food tested, the collaborators received 8 blind test samples consisting of a control sample and 3 levels of inoculated test sample, each in duplicate. The mean log counts for the methods were comparable for pasta filled with beef and cheese; frozen hash browns; cooked chicken patty; egg custard; frozen ground raw pork; and instant nonfat dried milk. The repeatability and reproducibility variances of the Petrifilm Rapid S. aureus Count Plate method were similar to those of the standard method.