Isolation of a mRNA encoding a glycine-proline-rich β-keratin expressed in the regenerating epidermis of lizard

Abstract

During scale regeneration in lizard tail, an active differentiation of β-keratin synthesizing cells occurs. The cDNA and amino acid sequence of a lizard β-keratin has been obtained from mRNA isolated from regenerating epidermis. Degenerate oligonucleotides, selected from the translated amino acid sequence of a lizard claw protein, were used to amplify a specific lizard keratin cDNA fragment from the mRNA after reverse transcription with poly dT primer and subsequent polymerase chain reaction (3′-rapid amplification of cDNA ends analysis, 3′-RACE). The new sequence was used to design specific primers to obtain the complete cDNA sequence by 5′-RACE. The 835-nucleotide cDNA sequence encodes a glycine-proline-rich protein containing 163 amino acids with a molecular mass of 15.5 kDa; 4.3% of its amino acids is represented by cysteine, 4.9% by tyrosine, 8.0% by proline, and 29.4% by glycine. Tyrosine is linked to glycine, and proline is present mainly in the central region of the protein. Repeated glycine-glycine-X and glycine-X amino acid sequences are localized near the N-amino and C-terminal regions. The protein has the central amino acid region similar to that of claw-feather, whereas the head and tail regions are similar to glycine-tyrosine-rich proteins of mammalian hairs. In situ hybridization analysis at light and electron microscope reveals that the corresponding mRNA is expressed in cells of the differentiating β-layers of the regenerating scales. The synthesis of β-keratin from its mRNA occurs among ribosomes or is associated with the surface of β-keratin filaments. © 2005 Wiley-Liss, Inc.