Purification, Characterization and Immobilization of Alginase Produced by Bacillus sp Associated with Sargassum wightii

Abstract

Seaweed sample was collected from tide pools and rock surfaces in theKanyakumari coastal region. The bacterial isolate was identified based on the morphological and cultural characteristics. The effect of different pH on alginase production by Bacillus sp. is at pH 8.0 (10.9± 0.34 IU/ml). Among the various temperatures tested, maximum alginase production was recorded in 40°C (15.9 ± 0.39 IU/ml). The effect of metal ions tested at calcium chloride (32.41 ± 0.47 IU/ml). Sodium alginate matrix was increased gradually up to 24hrs (0.53±0.012 IU/ml). Agar-Agar matrix was better steadily up to 24 hrs (0.6± 0.017 IU/ml).Gelatin matrix was increased gradually up to 24 hrs (0.37±0.10 IU/ml)There are four variables used for the factorial analysis including pH, temperature, alginate and ammonium chloride namely X1, X2, X3 and X4 respectively. There was a considerable variation in the alginase production depending on the four chosen variables. The maximum alginase production of 36.4 IU/ml was achieved by RSM (CCD).