Facile and Novel Synthesis of Spiky Gold Nanoparticles as an Efficient Antimicrobial Agent against Pseudomonas Aeruginosa

Abstract

The study aims to develop advanced antibacterial agents as nanoparticles instead of antibiotics due to the emergence of antimicrobial resistance. Pseudomonas aeruginosa is capable of causing many diseases, including severe bacterial pneumonia. There is a need for an efficient antibacterial agent to kill these pathogens. The objective of the study is to synthesize advanced antibacterial agents as nanoparticles for biomedical applications that can play a vital role in killing Gram-negative bacteria (Pseudomonas aeruginosa). A novel fabricated growth of hydrophilic spiky gold nanoparticles (SGNPs) via reduction method is reported. The surface plasmon resonance peak of the synthesized SGNPs was tuned under the near-infrared range. The SGNPs have anisotropic and spiky morphology with 68 nm size and -58 mV surface charge. They are pure, possessing adsorption similar to the organic material. Pseudomonas aeruginosa treated with synthesized SGNPs showed 60% bacterial death at the concentration of 100 μM. This work consists of the novel synthesis of SGNPs via a safe and simple reduction method. The synthesized SGNPs exhibit strong antibacterial activity against the Gram-negative bacteria Pseudomonas aeruginosa measured using a microplate assay test. The result showed that these SGNPs are ideal for biomedical applications.