Evaluation of in vitro antioxidant, antidiabetic activities and GC-MS analysis from Amomum nilgiricum leaf extract

Abstract

In the present study, hexane, ethyl acetate and methanol fraction of Amomum nilgiricum leaf was evaluated for antidiabetic efficacy through in vitro α-amylase and α-glucosidase assays, DPPH and H2O2 scavenging activities, followed by estimation of total phenol, total flavonoids and gas chromatography-mass spectrometry analysis. In the present study, a significant amounts of total phenolics (79.92±1.58 mg/g) and flavonoids (21.74± 0.89 mg/g) were showed from Ethyl acetae fraction. Ethyl acetate fraction showed maximum inhibition of DPPH radicals (82.31±2.33%) with IC50 value of 52 µg/ml and H2O2 scavenging activity (97.62±2.89%) with IC50 value of 78.57 µg/ml concentrations. The ethyl acetate fraction was revealed maximum α-amylase inhibition (77.23± 3.21%) with IC50 value 76.53 µg/ml. The ethyl acetate fraction recorded maximum α-glucosidase inhibition (85.36±2.58%) with IC50 value 79.54 µg/ml. Ethyl acetate fraction exhibited maximum inhibitory activity of glucose movement into outer solution across dialysis membrane at 250 µg/ml as compared to the control. The ethyl acetate fraction revealed maximum insulin secretory activity (130.5±3.66%) in RIN-m5F cells. Methanol fraction recorded maximum glucose uptake percent in yeast cells (67.08±1.68%) when compared to standard metronidazole (68.06±0.73%). The GC-MS analysis of ethyl acetate fraction was recorded the presence of six phytochemical constituents. This study scientifically validates the antidiabetic activity of A. nilgiricum. Hence, in view of its comparative hypoglycemic strength, it can work as a valuable healing agent in treating diabetes.