Salivary Pro-Inflammatory Cytokines and Salivary Bacterial Challenge Effect on Dental Caries: A Clinico-Molecular Cross-Sectional Study

Abstract

Objectives: Objective of this study is to determine the correlation between salivary cytokine concentrations and cariogram value high (C1) and low (C2) experience in people. In this direction, saliva cytokines showed a significant correlation with the patient's caries experience. Patients and Methods: Unstimulated whole saliva samples were collected from 38 appropriate populations of patients aged between 20 to 57 years old. All patients' DMF (decay/missing/filled) values were higher than 0. Twenty-one caries activity groups (C1) and 17 caries non-activity groups (C2) patients were collected for this study. Procedures of the clinical evaluation were the collection of saliva samples and oral examination. The whole saliva from 38 patients with C1 and C2 was investigated for the existence of GM-CSF, TNF alpha, IFN-g, IL-2, IL-4, IL-6, IL-8, and IL-10. Results: The study population consisted of 38 subjects with a mean age of 34.76 +/- 14.43 years. Likewise, there was a significant interaction between salivary flow rate, S. Mutans number, number of Lactobacillus, number of meals and IL-6, a significant difference in caries activity groups. Conclusion: Salivary cytokines demonstrated significant interactions to caries experience of the patient.