Improve In vitro Multiplication of Olive Shoots Using Environmental-Safe Chitosan, Selenium, and Silver Nanostructures

Abstract

Olive micropropagation has lately become a potent method for bulk multiplication. Olive trees are traditionally propagated commercially through stem cuttings or grafting. The current study aimed to examine the potential for some olive cultivars' in vitro growth and multiplication and the effectiveness of silver, chitosan, and selenium nanoparticles as microbial decontamination agents. Transmission electron microscopy carried out validation and characterization of the biosynthesized nanoparticles. The tested nanoparticles showed varying antimicrobial activity; when it came to preventing microbiological contamination, AgNPs were quite successful. The genotype substantially impacted shoot growth and multiplication rate; Koroneiki and Picual cultivars showed superior growth than Manzanillo cv. The development and multiplication rate of in vitro olive shoots was considerably altered by adding nanoparticles to the culture media. Silver nanoparticles reported greater values for shoot number, shoot length, leaf number, and multiplication rate than the chitosan and selenium nanoparticle treatments. The findings concluded that nanoparticles were effective in vitro disinfection agents and had a good impact on olive shoots' development and multiplication rate (especially AgNPs).