Abstract
StayGold is an exceptionally bright and stable fluorescent protein that is highly resistant to photobleaching. Despite favorable fluorescence properties, use of StayGold as a fluorescent tag is limited because it forms a natural dimer. Here we report the 1.6 Å structure of StayGold and generate a derivative, mStayGold, that retains the brightness and photostability of the original protein while being fully monomeric.
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CITATION STYLE
APA
Ivorra-Molla, E., Akhuli, D., McAndrew, M. B. L., Scott, W., Kumar, L., Palani, S., … Balasubramanian, M. K. (2024). A monomeric StayGold fluorescent protein. Nature Biotechnology, 42(9), 1368–1371. https://doi.org/10.1038/s41587-023-02018-w
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