THE IN VITRO RELEASE TESTING AND THE ANTIMICROBIAL ACTIVITY OF SEMI-SOLID DOSAGE FORMS WHICH CONTAIN SALICYLIC ACID

Abstract

The aim of the work was to study the effect of the base nature on the salicylic acid release from semi-solid dosage forms by an in vitro method followed by the quantitative determination using high-performance liquid chromatography (HPLC). It has been found that salicylic acid is released from the Vaseline base in the least amounts, in a higher manner from the oil/water (o/w) emulsion base, and its maximum release is from the water/oil (W/O) base. A direct relationship between the degree of release and the antimicrobial properties of the ointment, in relation to the reference strains of the American type culture collection (АТСС): Staphylococcus aureus АТСС 25293, Bacillus subtilis АТСС 6633, Escherichia coli АТСС 25922, Pseudomonas aeruginosa АТСС 27853 and Candida albicans АТСС 885-653, has been determined. It has been found that a more complete release of the salicylic acid from ointments on emulsion bases increases their antimicrobial properties compared to the Vaseline base. It has been shown that the active substance is highly soluble in oil, and the antimicrobial effect will be more pronounced when using an emulsion base of the second type (W/O).