Optimization of Fermentation Conditions for Ethanol Production from Renewable Biomass Using Response Surface Methodology

Abstract

Potato Peel Waste (PPW) is a potential lignocellulosic biomass substrate for bioethanol production due to its high starch content and easy availability. In this study, we performed research optimization of fermentation process by Response Surface Methodology (RSM) using Plackett-Burman design. The process herein included acid-base pre-treatment of biomass, which was then followed by enzymatic hydrolysis as a potential step. The concentration of reducing sugar in the hydrolysate thus obtained was then analyzed by DNSA method. After fermenting the hydrolysate with Saccharomyces cerevisiae for several days, distillation was done. Analysis of hydrolysate was done by FTIR. Pre-treatment is used for lignocellulosic biomass for improving the hydrolysis of the potato as it contains a high amount of cellulose and removal of lignin and hemicellulose. Cellulose converts into the reducing sugars and then to ethanol. Saccharification and fermentation methods were performed to acquire maximum yield of ethanol. The potato peels were pre-treated with Sulphuric acid and sodium hydroxide solutions.