Resin Replica in Enamel Deproteinization and its Effect on Acid Etching

Abstract

Purpose: The goal of this in vitro study was to identify the topographical features of deproteinized (NaOCl) and etched with phosphoric acid (H 3 PO 4) enamel surface, compared to phosphoric acid surface alone with a Resin Replica model. Materials: Ten extracted lower first and second permanent molars were polished with pumice and water, and then divided into 3 equal buccal sections having similar physical and chemical properties. The enamel surfaces of each group were subjected to the following treatments: Group A: Acid Etching with H 3 PO 4 37% for 15 seconds. Group B: Sodium Hypochlorite (NaOCl) 5.25% for 60 seconds followed by Acid Etching with H 3 PO 4 37% for 15 seconds. Group C; No treatment (control). All the samples were treated as follow: Adhesive and resin were applied to all groups after A, B and C treatment were performed; Then enamel/dentin decalcification and deproteinization and topographic SEM Resin Replica assessment were used to identify resin tags enamel surface quality penetration. Results showed that group B reached an area of 7. 52mm2 of the total surface, with a 5.68 mm2 (73%)resin tag penetration equivalent type I and II etching pattern, 1.71 mm2 (26%) equivalent to type III etching pattern and 0.07 mm2 (1%) unaffected surface. Followed by group A with 7.48 mm2 of the total surface, with a 3.47 mm2 (46 %)resin tag penetration equivalent to type I and II etching pattern, 3.30 mm2 (45 %)equivalent to type III etching pattern and 0.71 mm2, and (9 %) unaffected surface. Group C did not show any resin tag penetration. A significant statistical difference (P <0,001) existed between groups A and B in resin quality penetration, leading to the conclusion that when the enamel is deproteinizated with 5.25% NaOCl for 1 minute prior H 3 PO 4, the surface and topographical features of the replica resin penetration surface increases significantly with type I-II etching pattern.