On acid Phosphatase During Lens Regeneration in Triturus Pyrrhogaster (Boie)

Abstract

1. The material consisted of adult newts kept for 9 to 35 days following lentectomy and the condition of acid phosphatase in the lens during the regenerative process was investigated by GOMORI's improved technique. 2. Moderate acid phosphatase activity was demonstrated in the first half of regeneration with high reaction near the potential posterior pole of the lens in the course of differentiation to fiber cell. On the other hand, in the later half of regeneration, the activity decreased gradually with progress in differentiation and in the terminal stage of regeneration only a trace of reaction is noted in the lens epithelium. 3. In fiber cells, acid phosphatase appeared to be deposited in the GOLGI region. 4. Acid phosphatase activity paralleled the changes in nucleic acid, particularly RNA, and it is felt that it may play an important role in the metabolism of nucleoprotein in the cell accompanying cellular multiplication, differentiation and growth in the course of regeneration. © 1959, International Society of Histology and Cytology. All rights reserved.