Cytotoxic activity of green synthesis copper oxide nanoparticles using cordia myxa L. aqueous extract on some breast cancer cell lines

Abstract

The efficiency of copper oxide nanoparticles synthesized from aqueous extract of cordia myxa L. on some breast cancer lines AMJ-13,MCF-7, and HBL-100 as normal cell lines was studied. Different concentration of copper oxide nanoparticles (25,50,75,100) μg/ml at different times (24,48 and72) h were selected. The results showed that the effect of nanoparticles depend on the concentration. As the concentration of nanoparticles increase the percent of inhibition increase. It was found that concentration of copper oxide nanoparticles at 100 μg / ml gave the highest inhibitory rate of cell growth MCF-7 (71.1%) after 24 hours, while the percent of inhibition for AMJ-13 was (69.6)%. When the exposure time was increased to48 h, the rate of inhibition at 100 mg/ml was 80% for MCF-7 while 73% for AMJ13. By increasing the duration of the exposure to 72 hours, the rate of inhibition at l00μg/ml were (85.2 and 78.2)for MCF-7 and AMJ13 respectively. No significant inhibition was found for HBL-100 at different concentration and different times. These results was indicated that copper oxide nanoparticles synthesized from the aqueous extract of cordia myxa L. had a toxic effect on growth of some breast cancer cell lines.