Determinação do Teor de Tanino de Quatro Variedades de Sorgo por Intermédio de Métodos Quantitativos

Abstract

This experiment was conducted to compare the tannin content of four sorghum varieties using six quantitative methods of tannin determination. A completely randomized design in a 4 x 6 (variety x method) factorial arrangement with four replicates was used, where: T1: Folin Denis (Folin Denis reagent reflux); T2: Folin Denis (water reflux); T3: Folin Denis (water reflux followed by filtration); T4: Lowental, T5: Vanilin cloridric acid of Burns, and T6: Vanilin cloridric acid modified of Burns. The highest tannin content was observed when determined by Burns methods and the lowest content by Lowental method. The results showed a wide variability among the methods used to determine tannin. By the laboratory practice, the Polin Denis method - CNPSA may be the most rccomended method.