In vitro evaluation of methanolic extract of red seaweeds against α-amylase and α-glucosidase enzyme inhibitory activity

Abstract

Objective: In the present study, methanolic extract of red seaweeds, Spyridia filamentosa Grateloupia lithophila and , Hypnea musciformis against α- amylase, glucosidase enzyme inhibitory activity were carried out. Materials and methods: Phytochemical constituents of methanolic extract were qualitatively determined. Antidiabetic activity was evaluated by inhibitory potential of methanolic extract against α-amylase and α- glucosidase by spectrophotometric assays. Results: The crude methanolic extract shows stronger inhibitor effect to α- amylase and α- glucosidase of S. 50 filamentosa (IC = 58.02 and 66.06 µg/ml), G. lithophila (IC = 53.01 and 58.02 µg/ml) and H. musciformis (IC =50 50 48.01 and 51.02 µg/ml), respectively. Phytochemical screening of red seaweed extracts revealed the presence of phenols, protein, lipids and carbohydrates. Conclusion: The result suggest that crude methanolic extract of S. filamentosa have anti- diabetic potential through inhibition of - amylase and α- glucosidase.