Puerariae Flos extracts possess the potential antioxidant efficacy against oxidant stress

Abstract

In this study, yield of Puerariae Flos extract showed 60% ethanol gave the highest yield of 26.34%. Total polyphenol contents and total flavonoid contents were highest in 40% ethanol extract (5.86 mg TAE/g) and hot water extract (3.06 mg CE/g), respectively. Evaluation of the reducing power of transition metal ions such as phosphomolybdenum and ferric tripyridyltriazine complex were high in 100% ethanol extract (4.41 mg AAE/g) and hot water extract (42.62 µM TE/g), respectively. Evaluation of free radical scavenging activity such as DPPH, ABTS and NO were excellent in 40% ethanol extract (95.74 µM TE/g, 17.78 µM TE/g, 175.5 µg/mL) and the HP radical scavenging activity was excellent in 80% ethanol extract (308.3 µg/mL), respectively. A positive correlation (p < 0.01) was showed between TFC and FRAP assay (r = 0.866) highest significant. The correlation between PMA and ABTS radical scavenging activity was significantly high with r = 0.913 (p < 0.01). The overall results of this work showed that Puerariae Flos is a candidate natural source of antioxidants.