Age estimation in the harbour seal (Phoca vitulina) based on the closure of skull sutures and synchondroses

Abstract

Age estimation of individuals is an important tool for the management of marine mammals and is mostly done by microscopic analysis of growth layer groups (GLGs) in teeth (cementum and dentin) and bone. The present study evaluated the use of sutural and synchondrosal closure in the skull of the harbour seal (Phoca vitulina) as a potential non-destructive alternative for age estimation. For that, we scored the ectocranial closure of 12 selected sutures and synchondroses in a sample of 303 Eastern Atlantic harbour seals (P. v. vitulina; 138 males, 165 females), for which data on age at death were available, based on determined gross-morphological criteria in individuals younger than 1 year and cement layer analysis in canines in older individuals. A strong positive relationship between the sum of closure level scores (SCS) and age (males: r = 0.8797, females: r = 0.8825) was recorded, which was stronger than that for the relationship between age and condylobasal length (CBL, males: r = 0.7085, females: 0.7086) (all p-values <0.0001). In adult individuals (≥5 years), CBL was higher in males than females (p < 0.0001), while SCS did not significantly differ between the two sexes (p = 0.148). Our findings show that the analysis of sutural/synchondrosal closure is a valid alternative for age estimation in harbour seals, thereby confirming results of previous studies on other pinniped species.