Limited survey for aflatoxin contamination of polished rice imported into Japan

Abstract

Anal. of aflatoxins (AF) B1, B2, G1 and G2 in imported polished rice samples from Thailand, Pakistan and Bangladesh was performed by direct competitive ELISA (direct cELISA) and immunoaffinity column with high performance liq. chromatog. (IAC-HPLC) methods using monoclonal antibody. Direct cELISA method with the detection limit of 2.8 micro g/kg for AFB1 gave neg. results in all of 20 samples analyzed. On the other hand, using IAC-HPLC with the detection limit of 0.1 micro g/kg for each toxin, AFB1 was detected in 5 out of 20 (25 imported polished rice samples; in 3 Thailand and 2 Pakistan samples, with the concns. ranging 0.1 - 0.3 micro g/kg. In addn., AFB2 (0.1 micro g/kg) was detected in one of the Pakistan rice sample contaminated with AFB1 (0.3 micro g/kg). Even though the level of AF contamination in imported rice samples found to be well below the Japanese limit (10 micro g/kg), AF contamination in rice as a staple food of Asian is an important issue for evaluation of human exposure due to the frequency of intake. on SciFinder (R)