Phytochemical characterization and antioxidant potential of rustyback fern (Asplenium ceterach L.)

Abstract

Asplenium ceterach L. (syn. Ceterach officinarum Willd.) or rustyback fern is a plant species traditionally used in Mediterranean countries as an expectorant, diuretic, against spleen complaints, kidney stones and hemorrhoids. Phytochemical analysis of gametophytes and sporophytes of A. ceterach was performed , followed by comparative analysis of phenolic composition and the antioxidant properties of the extracts (scavenging capacities against ABTS •+ and DPPH •). Totally 16 phenolic compounds belonging to the classes of phenolic acids (hydroxybenzoic and hydroxycynnamic acids), flavonoids (flavan-3-ols, flavonols), and xanthones were identified using UHPLC/DAD/-HESI-MS/MS analysis. Different pheno-lics' composition of the two phases of the life cycle of this fern was observed which significantly determined their radical scavenging activities. In sporophytes of A. ceterach considerably high amounts of chlorogenic acid were found (~21 µg 100 mg-1 fresh weight), while xanthones were the most abundant in gametophytes (mangiferin glucoside, reaching 2.54 µg 100 mg-1 fresh weight), recommending this fern species as a valuable source of bioactive compounds.