Optimization of high-temperature bioethanol production from pineapple peel hydrolysate using response surface methodology

Abstract

This study aimed to determine the optimal conditions for bioethanol production from pineapple peels using response surface methodology at high temperatures. Evaluation of factors that affected on ethanol production at 40o C was carried out with 7 factors (urea, NH4NO3, MgSO4.7H2O, KH2PO4, yeast extract, pH, and yeast density) using the statistical model of Plackett-Burman Design. Optimizations of conditions for ethanol production were conducted with the response surface methodology using Central Composite Design. As a result, the optimal conditions of ethanol production from pineapple peel hydrolysate by S. cerevisiae YVN8 at 40oC were identified as followed: 0.30 g/L of NH4NO3, 0.64 g/L of yeast extract, and pH 4.41. In the optimal conditions, the ethanol concentration was attained at 33.3 g/L.