EHMTI-0267. Plasma anandamide concentration after aerobic exercise training in healthy individuals and episodic migraine patients

Abstract

Introduction: Anandamide (AEA) is an endocannabinoid operative in several biological functions. Nevertheless, it is not known the effect of aerobic exercise training (EXT) on plasma [AEA]. Aims: Because a dysfunctional endocannabinoid system has been suggested to underlie migraine (M) pathophysiology, we intended to explore the plasma [AEA] after EXT in M patients and healthy individuals. Methods: EXT protocol consisted of 12-week of supervised treadmill at standardized intensity, performed 3 times/week, 30 min./session. Four groups were separated for intervention or waiting list: Healthy subjects without AET (CC), healthy subjects undergoing EXT (CEXE), M patients without EXT (MC), and M patients undergoing EXT (MEXE). Patients had episodic migraine with and without aura (ICHDII). Blood collections were performed interictally at least 24h after attacks or anti-inflammatory use. AEA was quantified by LC/MS/MS. All participants took no preventive medication. Results: The study included 48 participants (12 for each group) and groups matched by age, sex, and BMI. Baseline [AEA] was not different between groups. AEA reduced in MEXE and CEXE, but was statistically significant only in CEXE (p = 0.007). There was a trend to a negative correlation between adherence and ΔAEA (r=-0.565, p = 0.056). Conclusions: Plasma AEA decreases after EXT in healthy subjects. In M patients, this response is prevented by lower adherence. Future studies should investigate the relationship between exercise-reward and AEA.∗p = 0.007, Friedman's Test.