MiR-373 suppresses gastric cancer metastasis by downregulating vimentin

Abstract

MicroRNA-373 (miR-373) has been reported to be an oncogene in a number of solid human tumors. However, the role of miR-373 in gastric cancer has not been completely elucidated and the mechanisms remain unclear. In the present study, we compared miR-373 expression between clinical gastric cancer tissues and paired non-tumorous tissues by reverse transcription-quantitative polymerase chain reaction. The impact of miR-373 on proliferation, migration and invasion in gastric cancer cells was additionally investigated. Hsa-miR-373 mimics were applied to mimic the function of endogenous miR-373. A colony formation assay and flow cytometry were performed to analyze the proliferation of gastric cancer cells. Wound healing and Transwell invasion assays were employed to detect the migratory and invasive abilities of gastric cancer cells. Western blotting was used to test the expression of epithelial-mesenchymal transition-associated proteins. The results demonstrated that the level of miR-373 in gastric cancer was upregulated compared with paired non-tumorous tissues. It was confirmed that miR-373 inhibited the migration and invasion of the gastric cancer cell lines SGC-7901 and HGC-27 by downregulating vimentin expression. The results of the present study demonstrated an oncogenic role of miR-373 in the metastasis of human gastric cancer, and may provide a novel therapeutic strategy for gastric cancer.