A new methodology to evaluate entomopathogenic fungi and formulated insecticides to control adults of aedes aegypti (Diptera: Culicidae)

Abstract

Summary The use of non-conventional insecticides and entomopathogenic fungi to control adult mosquitoes is increasing; however, the current methods are designed to evaluate the insecticidal action of the active ingredient. We describe the bioassay method to evaluate the effect of non-conventional insecticides and entomopathogenic fungi to control mosquitoes using the Potter tower. Twenty d after application, entomopathogenic fungi produced a proportion of mortality of 0.49 to 0.82, while non-conventional insecticides produced 100% mortality from 15 min after application. Our results showed that this bioassay could provide basic information on the effect of different non-conventional insecticides and entomopathogenic fungi on adult mosquitoes.