Exposure to low irradiances favors the synthesis of 9-cis β,β-carotene in Dunaliella salina (Teod.)

Abstract

We examined the effect of irradiance on the synthesis of β-carotene and its isomers by Dunaliella salina. Growth irradiance had a marked effect both on growth of the alga (which was suppressed at both low and high irradiances) and on the accumulation of β-carotene. The accumulation of β-carotene but not α-carotene was closely linked to an increase in irradiance. Growth at low irradiances (20-50 μmol m-2 s-1) promoted a high ratio of 9-cis to all-trans β-carotene (>2:1), while exposure to high irradiances (200-1,250 μmol m-2 s-1) resulted in a large reduction in this ratio (to <0.45:1). A similar pattern was seen for the geometric isomers of α-carotene, with exposure to low irradiance favoring the accumulation of the 9-cis form. The carotenoid biosynthesis inhibitors 4-chloro-5(methylamino)-2-(α-α-α-trifluoro-m-tolyl)-3-(sH)-pyridazinone and 2-(4-chlorophenylthio)triethylamine caused the accumulation of the precursors phytoene and lycopene, respectively, in D. salina. High-performance liquid chromatography and infrared analysis showed that phytoene adopted the 15-cis and all-trans forms (as in higher plants), and that lycopene primarily adopted the all-trans form. This indicates that isomerization of β-carotene takes place during or after cyclization.