Prevalence and distribution of antimicrobial resistance determinants of Escherichia coli isolates obtained from meat in South Africa

Abstract

Objective This study aimed to characterise antibiotics resistance of Escherichia coli isolates from the formal meat sector (FMS) and informal meat sectors (INMS). Method A total of 162 and 102 E. coli isolates from the FMS, and INMS respectively were isolated by standard culture-based, and biochemical reactions. The isolates were further confirmed by polymerase chain reaction (PCR). The disc diffusion method was used to screen for antimicrobial susceptibility against 19 different antibiotics. The presence of class 1-2 integrons in each E. coli isolates was assessed using 30-CS and 50-CS regions specific primers. Result Among the 19 antimicrobials, resistance to tetracyclines, aminoglycosides, cephalosporins, and nitrofurans were found to be more frequent than carbapenems and chloramphenicol. The number of multi-drug resistance ranged from three to ten antimicrobials. The resistant determinants with the highest prevalence in the FMS and INMS were; [aminoglycosides: AadA (40.6%; 31.9%), and strA (6.5%; 9.4%)], [β-lactams: AmpC (20%; 45%),], [Chloramphenicol: CatI (1.7%; 1.7%), and [tetracyclines: TetB (11.5%; 24%),], and [sulfonamides: Sul1 (22.2%; 26.7%),]. Conclusion Higher phenotypic resistance to cephalosporins and carbapenems were found in the FMS than in INMS. The multiple antibiotic resistance (MAR) indexes for FMS and INMS ranged from 0.2-0.5. The results reveal a high prevalence of multidrug-resistant E. coli isolates and resistance determinants, suggesting that consumers and handlers of such meat are at risk of contracting antibiotic-resistant E. coli-related foodborne disease.