Avian reovirus mRNAs are nonfunctional in infected mouse cells: translational basis for virus host-range restriction.

6Citations
Citations of this article
6Readers
Mendeley users who have this article in their library.

Abstract

Avian reovirus S1133 penetrates and uncoats in suspension cultures of mouse L cells. The multiple species of viral transcripts are produced in the cytoplasm of the infected cell, but they fail to associate with polysomes, consistent with the absence of viral protein synthesis. The selective block in avian virus mRNA translation is not overcome by coinfection with mammalian reovirus type 3, which replicates in mouse L cells, or by hypertonic shock or exposure to a low concentration of cycloheximide. Although the avian viral transcripts are inactive in vivo, RNA extracted from infected, nonpermissive L cells directs the synthesis of a normal spectrum of viral proteins in rabbit reticulocyte lysates. These results indicate that avian viral transcription is not restricted in mouse cells and that viral replication is prevented at the level of initiation of protein synthesis.

Cite

CITATION STYLE

APA

Benavente, J., & Shatkin, A. J. (1988). Avian reovirus mRNAs are nonfunctional in infected mouse cells: translational basis for virus host-range restriction. Proceedings of the National Academy of Sciences of the United States of America, 85(12), 4257–4261. https://doi.org/10.1073/pnas.85.12.4257

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free