Genetic and morphological characterization of the Aedes (Ochlerotatus) dorsalis (Diptera: Culicidae) group in North America

Abstract

An examination of the electrophoretically detectable variation among the North American members of the Aedes (Ochlerotatus) dorsalis group revealed large genetic differences among all 4 species. At least 9 of 18 loci examined (50%) were diagnostic for each species pair. However, morphological variation observed among species was low. Only Aedes canadensis (Theobald) was separated readily from the other members of this group [Aedes dorsalis (Meigen), Aedes melanimon Dyar and Aedes campestris Dyar & Knab] in all life stages. Characters traditionally used to separate the remaining 3 species were less reliable. In the adult female, Ae. melanimon may be distinguished from Ae. campestris by the scaling patterns of the wings and abdomen, but Ae. dorsalis could not be distinguished reliably by these characters. Adults of Ae. dorsalis may be separated reliably from those of Ae. campestris and Ae. melanimon only by the length of the subapical tooth relative to the length of the tarsal claw. Ae. melanimon was identified in the larval stage by the short mesothoracic hair 1. Eight larval characters differed between Ae. dorsalis and Ae. campestris. However, the ranges of these characters overlapped and no character was truly diagnostic. Genetic variation within species was low as measured by average heterozygosity and Nei's genetic distance coefficients. No allozymes were diagnostic for coastal and inland populations of Ae. dorsalis, and the pattern of genetic differentiation within this species did not correspond to the geographic location of the populations examined. Therefore, the genetic data did not support the hypothesis that Ae. dorsalis represents a complex of 2 or more cryptic species.