Mapping of the eibi1 gene responsible for the drought hypersensitive cuticle in wild barley (Hordeum spontaneum)

Abstract

Segregation analysis showed that eibi1, a drought hypersensitive cuticle wild barley mutant, was monogenic and recessive, and mapped in two F2 populations, one made from a cross between the mutant and a cultivated barley (cv. Morex), and the other between the mutant and another wild barley. A microsatellite marker screen showed that the gene was located on barley chromosome 3H, and a set of markers already assigned to this chromosome, including both microsatellites and ESTs, was used to construct a genetic map. eibi1 co-segregated with barley EST AV918546, and was located to bin 6. The synteny between barley and rice in this region is incomplete, with a large discrepancy in map distances, and the presence of multiple inversions.