Carbon nanotubes-based potentiometric bio-sensors for determination of urea

Abstract

The possibility of using disposable plastic-carbon potentiometric sensors as enzyme biosensors was examined. Urease enzyme was immobilized on poly(vinyl chloride) based H+- or NH4+-selective membranes using cellulose acetate. This approach has resulted in a potentiometric response on changing the pH of the solution or NH4+ ion content due to an enzymatic reaction that occurs between urease and urea. Both types of potentiometric biosensors for urea were characterized by good analytical parameters as high sensitivity and fast response time.