A stability indicating HPLC method for the simultaneous determination of valsartan and ramipril in binary combination

Abstract

A simple, sensitive and validated HPLC method has been developed to determine valsartan and ramipril simultaneously in synthetic mixture. Chromatographic separation was achieved on a C-18 column using a mixture of acetonitrile and water in the ratio 55:45 (v/v), pH adjusted to 3.6 with 88% orthophosphoric acid at a wavelength of 215 nm. Linearity of the method was found to be in the concentration range of 50-250 μg/ml for valsartan and100-500 μg/ml for ramipril with correlation coefficient greater than 0.999. The total eluting time for the two components is less than five minutes. The method can be used for simultaneous determination of valsartan and ramipril.