An effective dispersive liquid-liquid microextraction method for pharmaceutical extraction: Optimization via central composite design

Abstract

In this study, a simple and efficient Dispersive Liquid-Liquid Microextraction (DLLME) was developed to remove diclofenac sodium (DF) from water samples. Various parameters such as diclofenac concentration in aqueous phase (10 50 mg/L), process time (2 10 min), extraction solvent concentration (0:005 0:025 M), and centrifuge speed (1000 5000 rpm) were investigated. The experimental design was performed using the Response Surface Methodology (RSM), which is based on the central composite design, to reduce the number of experiments and determine the optimal extraction conditions. The effect of the single and simultaneous operational parameters was evaluated. In this regard, the extraction concentration of 0.01 M, the initial diclofenac concentration in the aqueous phase 20 mg/L, the rotation speed of the centrifuge at 4000 rpm, and the residence time of 8 min were obtained as the optimum operating conditions. According to the other studies and available findings, the ratio of two-phase organic and aqueous discharges (Qorg/Qaq) was set to 1:9. The diclofenac extraction from the aqueous phase was 77.91% in optimum operating conditions.