ESIPT-based fluorescence probe for the rapid detection of peroxynitrite ‘AND' biological thiols

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Abstract

An ESIPT-based ‘AND' logic fluorescence probe (GSH-ABAH) was developed for the simultaneous detection of ONOO− and biological thiols. GSH-ABAH was shown to have good cell permeability and with the addition of just SIN-1 (ONOO− donor) or GSH, no fluorescence response was observed in live cells. However, in the presence of both analytes GSH-ABAH could be used to image exogenous ONOO− ‘AND' GSH added to RAW264.7 cells.

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Wu, L., Han, H. H., Liu, L., Gardiner, J. E., Sedgwick, A. C., Huang, C., … James, T. D. (2018). ESIPT-based fluorescence probe for the rapid detection of peroxynitrite ‘AND’’ biological thiols.’ Chemical Communications, 54(80), 11336–11339. https://doi.org/10.1039/c8cc06917d

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