Identification of Potentially active Methanogenic Population for Degradation of Organic Substances in UASFFR for Treating Sago Wastewater

Abstract

Anaerobic digestion process is the best method for treating industrial wastewater with dynamic relationship between microorganisms. The objective of this research work is to assess the microbial population that coordinates with anaerobic codigestion of biomass cultivated on sago wastewater. An upflow anaerobic sludge fixed film reactor was used to achieve high rates of microbial decomposition. The bacterial colonies were counted and predominant culture was streaked in newer sterile nutrient agar plate. The predominant culture was subjected to molecular identification using 16S rRNA gene sequencing and Gram’s staining. Isolated organism chryseomicrobium species and the organism are identified as Chryseomicrobium palamuruense by using the biochemical and 16S rRNA gene sequencing.