Crystallization and preliminary X-ray analysis of a C-terminal TonB fragment from Escherichia coli

Abstract

The TonB protein located in the cell wall of Gram-negative bacteria mediates the proton motive force from the cytoplasmic membrane to specific outer membrane transporters. A C-terminal fragment of TonB from Escherichia coli consisting of amino-acid residues 147-239 (TonB-92) has been purified and crystallized. Crystals grew in space group P21 to dimensions of about 1.0 x 0.12 x 0.12 mm. A native data set has been obtained to 1.09 Å resolution. © 2004 International Union of Crystallography.