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Single-feature polymorphism discovery in the barley transcriptome.

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Abstract

A probe-level model for analysis of GeneChip gene-expression data is presented which identified more than 10,000 single-feature polymorphisms (SFP) between two barley genotypes. The method has good sensitivity, as 67% of known single-nucleotide polymorphisms (SNP) were called as SFPs. This method is applicable to all oligonucleotide microarray data, accounts for SNP effects in gene-expression data and represents an efficient and versatile approach for highly parallel marker identification in large genomes.

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Rostoks, N., Borevitz, J. O., Hedley, P. E., Russell, J., Mudie, S., Morris, J., … Waugh, R. (2005). Single-feature polymorphism discovery in the barley transcriptome. Genome Biology, 6(6). https://doi.org/10.1186/gb-2005-6-6-r54

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