Prevalence of Very Low Numbers of Potential Pathogenic Isolates of Yersinia enterocolitica and Yersinia intermedia in Traditional Fast Foods of India

Abstract

In this study, an incidence pattern of 1. 7% for Yersiniaenterocolitica and 2. 5% for Y. intermedia were observed in an analysis of 120 diversified food samples collected from the local market of Mysore, Southern India. Two native isolates characterized as Y. enterocolitica belonged to biotype 1B and revealed the presence of major virulence related traits such as regulator of virulence, mucoid Yersinia factor regulator, attachment invasion locus, heat stable enterotoxin, Yersinia type II secretory system and phospholipase A in PCR. Force type neighbor-joining phylograms generated for Y. enterocolitica based on PCR amplicons of rovA and ypl showed 100% homology with two to three strains of Y. enterocolitica and about 75% homology with several strains of Y. pestis. © 2011 Association of Microbiologists of India.