Preliminary study on overproduction of reactive oxygen species by neutrophils in diabetes mellitus

Abstract

AIM: To assess the amount and pattern of reactive oxygen species (ROS) production in diabetic patient-derived neutrophils. METHODS: Blood samples from type 2 diabetes mellitus (DM) patients and volunteers (controls) were subjected to neutrophil isolation and the assessment of neutrophil oxidative burst using chemiluminescence assay. Neutrophils were activated by using phorbol myristate acetate (PMA) and neutrophils without acti-vation were kept as a negative control. The chemilu- 7|Issue 13| WJD|www.wjgnet.com Ridzuan N et al . ROS overproduction by neutrophils in DM minescence readings were obtained by transferring cell suspension into a 1.5 mL Eppendorf tube, with PMA and luminol. Reaction mixtures were gently vortexed and placed inside luminometer for a duration of 5 min. RESULTS: Our results showed that in the resting condition, the secretion of ROS in normal non-diabetic individuals was relatively low compared to diabetic patients. However, the time scale observation revealed that the secreted ROS declined accordingly with time in non-diabetic individuals, yet such a reduction was not detected in diabetic patients where at all the time points, the secretion of ROS was maintained at similar magnitudes. This preliminary study demonstrated that ROS production was significantly higher in patients with DM compared to non-diabetic subjects in both resting and activated conditions. CONCLUSION: The respiratory burst activity of neutrophils could be affected by DM and the elevation of ROS production might be an aggravating factor in diabetic-related complications. Core tip: This is a preliminary study that investigates the activation status of peripheral blood-derived neutrophils in type 2 diabetes. This study clearly indicated that the neutrophils from type 2 diabetic patients are highly activated upon in vitro stimulation and hence produce greater amounts of reactive oxygen species (ROS) compared to a normal individual. Release of a greater volume of ROS could serve as an additional risk for end organ injury in type 2 diabetes mellitus. Ridzuan N, John CM, Sandrasaigaran P, Maqbool M, Liew LC, Lim J, Ramasamy R. Preliminary study on overproduction of reactive oxygen species by neutrophils in diabetes mellitus. World J Diabetes 2016; 7(13): 271­278 Available from: URL: