Study of Binahong Leaves ( Anredera cordifolia (Ten.) Steenis) Potency as an Herbal Feed Additive for Lactating Dairy Animals

Abstract

This study aims to investigate the potential of binahong leaves (Anredera cordifolia (Ten.) Steenis) as an herbal feed additive to address subclinical mastitis in lactating dairy animals. The extracted leaves were collected through a 95% ethanol maceration process, and the phytochemical content was subsequently analyzed qualitatively and quantitatively using Thin Layer Chromatography (TLC) and UV-visible spectrophotometry. Furthermore, the antibacterial potential was tested with extract concentrations ranging from 5% to 30% using the zone of inhibition method against bacteria causing subclinical mastitis, such as Streptococcus agalactiae, Staphylococcus aureus, and Staphylococcus epidermidis. The data were analyzed descriptively. The research findings indicate that binahong leaves extract contains polyphenols and saponins, supported by quantitative results, namely, 6.11 mg total phenols, 71.80 mg total flavonoids, 2.22% total saponins, 3.81% total tannins, and 233.33 μg/mL antioxidants. In 10-15% extract concentration, an average inhibition zone of approximately 10 mm was achieved. It can be concluded that binahong leaf extract at concentrations of 10-15%, containing high levels of flavonoids and antioxidants, has strong potential as an herbal feed additive to address subclinical mastitis in lactating dairy animals.