BsiY I, a novel thermophilic restriction endonuclease that recognizes 5′ CCNNNNNNNGG 3′ and the discovery of a wrongly sequenced site in pACYC177

Abstract

A new type II restriction endonuclease designated BsPi I has been purified from a thermophilic soil Bacillus stearothermophllus strain. This enzyme recognizes and cleaves the highly degenerate sequence 5′ CCNNNNNINNGG 3′. During the Identification of the recognition sequence of BsiY I, we discovered that there should be five G nucleotldes instead of four at position 1227-1230 of the plasmld pACYC177. © 1991 Oxford University Press.