Description of correlation between quantitative and qualitative assays on candlenut DNA

Abstract

DNA isolation is a crucial step in the molecular analysis, which influences the quantity and quality of the DNA. The objective of this study was to determine the correlation between DNA quantitative and qualitative assays on Candlenut (Aleurites moluccana) DNAs. This study was carried out in February up to April 2019. The research procedures consisted of sample collection, DNA isolation using CTAB method and Qiagen DNeasy Plant Mini Kit (QDPMK kit protocol), DNA quantitative assay using Qubit 3.0 fluorometer, and DNA qualitative assay using horizontal electrophoresis. No positive correlation was detected between DNA quantitative and DNAqualitative assays. The quantitative assay observed that the isolated DNA using QDPMK yielded more DNAs than CTAB (<0.2 ng/l). Meanwhile, DNA qualitative assay reported QDPMK had better DNA quality than CTAB