Evaluation of blood glucose, hepatic biomarkers and histopathology of alloxan induced diabetic rats fed Sesamum indicum compounded diet

Abstract

This study was aimed at evaluating the blood glucose level, hepatic biomarkers and histology of alloxan induced diabetic rats fed with Sesamum indicum compounded diet. Thirty-Six male albino rats were divided into nine groups (normal control, diabetic control, standard drug-glibenclamide treated, diabetic rats fed with 15%, 30% and 60% Sesamum indicum compounded diet and normal rats fed with 15%, 30% and 60% Sesamum indicum compounded diet and induced with diabetics after 14 days) of four rats each and the study lasted for 21 days. The induction of diabetes was done by a single dose of freshly prepared alloxan (120 mg/kg b.wt.) given intraperitoneally. The level of blood glucose was determined using Acu-check glucometer while the hepatic biomarkers was determined using standard spectrophotometric method. The blood glucose of the groups fed with the diet were observed to reduce when compared with the diabetic untreated group. Significant (p<0.05) reduction was observed for the hepatic biomarkers when compared with diabetic control. From the histopathology of the liver, the results showed improved liver for the groups fed with the compounded diet when compared with the diabetic control group that showed a damaged liver. The results of this study have shown that Sesamum indicum has both protective and ameliorative effect on blood glucose, hepatic biomarkers and restore the distorted liver of a diabetic rat.