ATP Prevents both Hydroperoxide‐Induced Hydrolysis of Pyridine Nucleotides and Release of Calcium in Rat Liver Mitochondria

Abstract

The mechanism of the hydroperoxide‐induced release of calcium from rat liver mitochondria was investigated. In calcium‐loaded mitochondria both the hydroperoxide‐induced hydrolysis of pyridine nucleotides and the release of calcium is inhibited by intramitochondrial ATP. In submitochondrial particles, in the absence of ATP a hydrolytic product of NAD+, probably ADP‐ribose, binds covalently to a protein of the inner mitochondrial membrane. It is proposed that a compound deriving from NAD(P)+ is an important factor in the calcium release mechanism in rat liver mitochondria. Copyright © 1981, Wiley Blackwell. All rights reserved