Abstract
Using Nα,Nα-bis(carboxymethyl)lysine to react with FePt magnetic nanoparticles, we synthesized the FePt?NTA conjugate, which immobilizes Ni2+ ions and selectively binds to histidine-tagged proteins at concentration as low as 0.5 pM. This simple system serves as a useful alternative to existing protocols for protein separation and also acts as a versatile agent for transporting and anchoring proteins. Copyright © 2004 American Chemical Society.
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CITATION STYLE
Xu, C., Xu, K., Gu, H., Zhong, X., Guo, Z., Zheng, R., … Xu, B. (2004). Nitrilotriacetic Acid-Modified Magnetic Nanoparticles as a General Agent to Bind Histidine-Tagged Proteins. Journal of the American Chemical Society, 126(11), 3392–3393. https://doi.org/10.1021/ja031776d
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