Study on Induction and Culture of Callus of Citrus grandis cv. 'Hongmianmiyou'

Abstract

Citrus grandis cv. 'Hongmianmiyou' is mutated from 'Guanximiyou', which has been widely cultivated in Sichuan Province because of the attractive appearance, rich nutritional ingredients and trace elements. To effectively cultivate more Hongmianmiyou seedlingsfor production, we established the callus tissue induction system. The basic nutrient medium consisted of MS medium with 3% of sucrose and 0.8% of agar, adjusted to pH 5.8.Various concentrations of 6-benzylaminopurine and 2,4-dichlorophenoxyacetic, as well as polyvinyl pyrrolidone and active carbon, and culture condition were carried out to developprotocols for the in vitro propagation of pummelo. The appropriate formula was MS mediumsupplemented with 1 mg•L-1of 2,4-D,0.5 mg•L-1of 6-BAand 0.05% of active carbon. Explants were cultured maintained under a 16 h photoperiod provided by cool white fluorescent light 1200 Lux after cultured at dark for 7 days. Light green compact callus induced from semi-lignified stems explant of spring shoots in 'Hongmianmiyou', reaching 97% induction rate.