A Dietary Blend of Synbiotic and Organic Acids has been Demonstrated to be Beneficial for Nile Tilapia Fingerlings

Abstract

Zootechnical additives are widely used in animal diets to enhance health and performance throughout different production stages. This study evaluated the effects of a blend of synbiotic and organic acids on Nile tilapia fingerlings. The investigation assessed growth performance, enzyme activity, proximate composition, and tissue histology. A total of 160 fish (initial weight: 2.22 (Formula presented.) 0.06 g) were assigned to five treatments in a randomised design with four replicates for 31 days, fed a 45% crude protein diet. The blend was incorporated into the feed via oil coating (2%) at dosages of 0.0 g kg (Formula presented.) (control), 2.50, 5.00, 7.50, and 10.0 g kg (Formula presented.). Although the blend did not affect growth performance, it modified body composition and improved physiological health indicators. Fish fed 2.50 g kg (Formula presented.) showed higher moisture and lower protein and mineral content (P (Formula presented.) 0.05). Digestive enzyme activity increased at 5.00 g kg (Formula presented.), whilst antioxidant enzymes (SOD and CAT) were enhanced at 7.50 and 10.0 g kg (Formula presented.) (P (Formula presented.) 0.05). These results suggest that higher blend levels (5.00–10.0 g kg (Formula presented.)) strengthen metabolic and antioxidant responses, highlighting the blend's potential to improve fish physiological health and support sustainable aquaculture.