Antioxidant and antifungal activities of collagen hydrolysates from skin of milkfish (Chanos chanos) hydrolyzed using various bacillus proteases

Abstract

The skin of fish contains collagen which can be used as a material for food, pharmacy or cosmetics. Collagen from fish is a good source of bioactive peptides which are produce through enzymatic hydrolysis and may be beneficial for human health. This research was conducted to investigate antioxidant and antifungal activities of milkfish (Chanos chanos) collagen which was hydrolyzed by Bacillus licheniformis and Bacillus thuringiensis proteases. The collagen was isolated from milkfish skin and the hydrolysis was done using B. licheniformis protease and crude protease from B. thuringiensis at alkaline condition, 55°C for 30 and 60 minutes. The hydrolysates obtained were assayed for antioxidant activities using ABTS and DPPH methods and antifungal activities against Candida albicans. The results showed that generally, collagen hydrolyzed using B. thuringiensis protease both 30 and 60 minutes showed higher antioxidant activities than of that hydrolyzed using B. licheniformis protease. The hydrolysates also reduced C. albicans at least for 5 log cycles. The results indicated that collagen hydrolyzed using B. thuringiensis protease is very potent as antioxidant and antifungal against C. albicans in future pharmaceutical product.