Genomic variations in the 2'-5' oligoadenylate synthetase 1 (OAS1) gene in zebu cattle and its crossbreds of Indian origin

Abstract

In the antiviral host defense mechanisms, the role of mammalian OAS/RNASEL pathway is very significant. These enzymes are interferon-inducible and activated by binding to double-stranded RNA (dsRNA) which are present in virus infected cells. The OAS proteins functions through its receptor, the 2-5Adependent ribonuclease (RNaseL) and activated OAS-RNaseL system degrades viral and cellular RNA and subsequently inhibits protein synthesis. Polymorphisms in the human and equine OAS gene cluster have been previously utilized for casecontrol analysis of virus-induced disease. But no polymorphisms have yet been identified in the bovine OAS1 genes for use in similar case-control studies. The promoter and coding regions of the OAS1 gene was amplified and screened for polymorphisms by PCR-SSCP and sequencing in Sahiwal and Frieswal animals. Two SNPs have been identified in the promoter region of OAS1 gene, which have predicted to create/delete sites for transcription factors. Specific amplification of the exonic regions of the OAS1 gene have identified 26 SNPs and one dinucleotide repeats, among them 14 are mis-sense variants. These polymorphisms are the first to be reported in OAS1 gene and will facilitate future case-control studies of cattle susceptibility to infectious diseases.